Study on Optimum Extraction Conditions for Olive Leaf Extracts Rich in Polyphenol and Flavonoid

ŞAHİN SEVGİLİ S., İlbay Z., KIRBAŞLAR Ş. İ.

Separation Science and Technology (Philadelphia), vol.50, no.8, pp.1181-1189, 2015 (SCI-Expanded) identifier

  • Publication Type: Article / Article
  • Volume: 50 Issue: 8
  • Publication Date: 2015
  • Doi Number: 10.1080/01496395.2014.966203
  • Journal Name: Separation Science and Technology (Philadelphia)
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.1181-1189
  • Keywords: flavonoid, Olea europaea, optimization, polyphenol, RSM, ultrasound-assisted extraction
  • Uşak University Affiliated: Yes

Abstract

Ultrasound-assisted extraction (UAE) of polyphenols and flavonoids from olive leaves was investigated. The effects of temperature (27−37°C), solvent concentration (10−70% ethanol (EtOH), v/v) and time (30−60 min) were determined by both experimental and Response Surface Methodology (RSM) techniques. Free radical scavenging activity for the antioxidant capacity was tested by 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical. For total phenolic content (TPC), 43.8252 mg-GAE/g dried leaf was predicted at the optimum conditions (34.18°C, 43.61% (v/v) of EtOH, and 59.99 min). In case of total flavonoid content (TFC), 31.9920 mg-CE/g dried leaf was calculated at the optimum conditions (34.44°C, 70% (v/v) of EtOH, and 60 min).