The effects Metformin/Irinotecan-loaded PLGA nanoparticles on glutamate re-uptake time and alteration EAAT1 gene expression level in vitro

TAGHIZADEHGHALEHJOUGHI A., HACIMÜFTÜOĞLU A., ÇETİN M., Kaplan A. B. U., Butuner S., TAŞPINAR N., ...Daha Fazla

Nanomedicine Journal, cilt.6, sa.1, ss.35-42, 2019 (Scopus) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 6 Sayı: 1
  • Basım Tarihi: 2019
  • Doi Numarası: 10.22038/nmj.2018.06.005
  • Dergi Adı: Nanomedicine Journal
  • Derginin Tarandığı İndeksler: Scopus
  • Sayfa Sayıları: ss.35-42
  • Anahtar Kelimeler: EAAT1, Irinotecan, Metformin, PLGA, Voltammetry
  • Uşak Üniversitesi Adresli: Evet

Özet

Objective(s): The present study was designed to evaluate of Metformin/Irinotecan-loaded poly-lactic-coglycolic acid (PLGA) nanoparticles (NPs) effects on glutamate re-uptake time and receptor expression status in both glioblastoma multiforme (GBM) and cortex neuron cultures. The study was performed on glioblastoma cell line and primer cortex neuron. Materials and Methods: The re-uptake time and gene expression status of pure drugs and MET- or IRIloaded- PLGA NPs on healthy neuron cells and U-87 MG cell line were investigated by using glutamate specific voltammetry electrodes technique and real time PCR. Results: Both MET and MET-PLGA NPs (1 and 2 mM) exhibited significant cytotoxicity on both U87MG and neuron cells. MET and MET-PLGA NPs (0.5 mM) showed lower cytotoxic effects on both cells. IRI and IRI-PLGA NPs (100 μM) had significant cytotoxic effects on both cell lines. Conclusion: All drug-loaded NPs caused a significant reduction in glutamate reuptake time compared with free drugs, blank NPs and cancer cells control groups. Consequently, MET- and IRI-loaded PLGA NPs may be a promising approach to treat GBM.