In Silico evaluation of phytoconstituents from Carica Papaya and its anti-hyperglycemic activities on high sucrose-induced oxidative stress in Drosophila melanogaster
Scientific Reports, cilt.15, sa.1, 2025 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 15 Sayı: 1
- Basım Tarihi: 2025
- Doi Numarası: 10.1038/s41598-025-13246-2
- Dergi Adı: Scientific Reports
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, Chemical Abstracts Core, MEDLINE, Veterinary Science Database, Directory of Open Access Journals
- Anahtar Kelimeler: Carica Papaya, Diabetes, Drosophila melanogaster, In Silico, Oxidative stress
- Uşak Üniversitesi Adresli: Evet
Özet
Oxidative stress plays a key role in the development of metabolic disorders, such as diabetes. This study investigated the phytoconstituents present in Carica papaya (AECP) using an in silico model, and its anti-hyperglycemic activities on high sucrose-induced oxidative stress in Drosophila melanogaster. In silico molecular docking was performed to analyze the binding potential of AECP’s bioactive compounds to key diabetes targets. Flies were fed a diet containing 30% sucrose to induce oxidative stress, followed by administration of AECP at doses of 50 and 100 mg/kg for five days. Biochemical assays assessed were glucose, total thiols, catalase, glutathione S-transferase (GST), and nitric oxide. In silico analysis revealed that carpaine, myricetin 3-rhamnoside, orientin 7-O-rhamnoside, and quercetin in AECP exhibited strong binding potential to key diabetes targets (alpha-amylase, beta-glucosidase, dipeptidyl peptidase 4, PPARG, and SGLT-2)). In fruit flies, sucrose-diet significantly (p < 0.05) reduced total thiol level, and catalase and GST activities while increasing glucose and nitric oxide levels. The AECP in a dose-dependent manner significantly (p < 0.05) reversed these changes, demonstrating its antioxidant and possible anti-hyperglycemic properties. These findings suggest that AECP may be a potential therapeutic agent for mitigating oxidative stress and supports its potential use in managing diabetes.