IN VITRO REGENERATION OF LENTIL (Lens culinaris); PROTOCOLS AND CHALLENGES

Küplemez H., Yıldırım M. U., Sarıhan E. O.

ANKARA INTERNATIONAL CONGRESS ON SCIENTIFIC RESEARCH-IX, Ankara, Türkiye, Ankara, Turkey, 26 - 28 December 2023, pp.1115-1120, (Full Text)

  • Publication Type: Conference Paper / Full Text
  • City: Ankara
  • Country: Turkey
  • Page Numbers: pp.1115-1120
  • Uşak University Affiliated: Yes

Abstract

  • Grain legumes (Fabaceae) have a lot of uses in human nutrition owing to their protein-rich seeds. To enhance grain legume production and yield per unit area, new varieties are needed to developed and spread. Under such conditions, grain legumes have a significant potential to adopt biotechnological approaches for improving grain legumes, such as tissue culture. Fabaceae species are difficult to regenerate in vitro, are mainly recalcitrant and frequently exhibit high genotypic specificity. Grain legumes have lower regeneration potential than forage legumes. Plant regeneration from somatic cell in vitro culture has proven challenging in large seeded leguminous species, as opposed to forage legumes. Morphogenesis is exceedingly slow in them, and associated concerns such as the production of albinos, vitreous tissues, and no-response in dedifferentiated calli pose major challenges to the employment of in vitro techniques in legume improvement. Within those species, the lentil was widely studied in tissue culture experiments to conduct an efficient propagation and breeding methods. There has a significant potential to adopt biotechnological technologies for improving and lentils, such as tissue culture. However, due to its recalcitrant disposition in in vitro environment, cultivar improving through biotechnology approaches appears to be limited and difficult to regenerate. Numerous prior research have been conducted in order to construct successful tissue regeneration systems in vitro conditions and to investigate the possibilities of adopting biotechnology to lentil development initiatives. In this study, in vitro regeneration protocols and difficulties of lentil plant have been investigated.