Akademik Veri Yönetim Sistemi
Medical records-international medical journal (Online), cilt.6, sa.1, ss.89-94, 2024 (Hakemli Dergi)
Aim: Sonodynamic antitumor therapy is a promising, novel method for the treatment of cancer. To determine the effects of malachite
green (MG) in the presence of ultrasound (US), MG was tested in vitro on HL60 cells at different concentrations as a sonodynamic
compound. We investigated cell viability, morphology, and the occurrence of endoplasmic reticulum (ER) stress after MG-mediated
sonodynamic therapy (SDT) in HL60 cells.
Material and Method: Four groups were formed, including a control group, a group subjected to ultrasound (US) only, a group treated
with various concentrations of MG, and a group treated with US using the same concentrations. The cells were treated with 1MHz
ultrasound at 2 W/cm2
for 3 minutes. The assessment of cell viability was conducted 24 hours post-treatment through the utilization
of the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Cell morphology and apoptotic index were
determined using Giemsa staining, while GRP78 and PERK expressions were determined through immunocytochemistry staining.
Results: The cell cytotoxicity of HL60 cells significantly increased after MG-mediated sonodynamic therapy. After treatment,
apoptotic cells with micronuclei were observed morphologically. Significant levels of GRP78 and PERK expression were observed in
all groups, except for PERK expression in the US group, compared to the control group.
Conclusion: The induction of ER stress, accompanied by intense apoptosis and a marked decrease in cell viability, demonstrates the
potential of MG-mediated sonodynamic therapy in cancer treatment. Investigating ER stress as a molecular target may contribute
to improving the treatment method.
Keywords: HL60 cells, malachite green, sonodynamic therapy, apoptosis, ER stress