In vitro effect of hyoscine-N-butyl bromide and diclofenac sodium in human tuba uterina

Sengul M., Karadas B., Acar-Sahan S., Ozturk O., Yılmaz H., Simsek F., ...More

Basic and Clinical Pharmacology and Toxicology, vol.135, no.2, pp.173-179, 2024 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 135 Issue: 2
  • Publication Date: 2024
  • Doi Number: 10.1111/bcpt.14038
  • Journal Name: Basic and Clinical Pharmacology and Toxicology
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, PASCAL, BIOSIS, CAB Abstracts, Chemical Abstracts Core, Chimica, EMBASE, Environment Index, MEDLINE, Veterinary Science Database
  • Page Numbers: pp.173-179
  • Keywords: diclofenac sodium, fallopian tube, hyoscine-N-butyl bromide, in vitro, tubal occlusion
  • Uşak University Affiliated: No

Abstract

Introduction: To investigate the in vitro effect of diclofenac on tubal smooth muscle as an alternative to hyoscine-N-butyl bromide, which is used for premedication before hysterosalpingography (HSG). Material and Methods: Fallopian tubes were retrieved from seven healthy women after bilateral tubal ligation and in vitro contractility and histological studies were conducted using tissue bath and immunohistochemistry. Results: Diclofenac sodium and hyoscine-N-butyl bromide did not significantly change the basal mean tension; however, they decreased the contractions induced by potassium chloride (KCl). The relaxant effect of diclofenac sodium and hyoscine-N-butyl bromide was not statistically significantly different. The presence of cyclooxygenase (COX)-2 enzyme in the fallopian tube was demonstrated by immunohistochemical studies. Conclusions: The in vitro relaxant effect of diclofenac sodium on the fallopian tube is similar to hyoscine-N-butyl bromide. Diclofenac may have the potential to be used as an alternative to hyoscine-N-butyl bromide in premedication in HSG.