Cytotoxic and genotoxic effects of clopyralid herbicide on Allium cepa roots
Environmental Science and Pollution Research, cilt.28, sa.35, ss.48450-48458, 2021 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 28 Sayı: 35
- Basım Tarihi: 2021
- Doi Numarası: 10.1007/s11356-021-13994-4
- Dergi Adı: Environmental Science and Pollution Research
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, IBZ Online, ABI/INFORM, Aerospace Database, Aqualine, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, EMBASE, Environment Index, Geobase, MEDLINE, Pollution Abstracts, Veterinary Science Database, Civil Engineering Abstracts
- Sayfa Sayıları: ss.48450-48458
- Anahtar Kelimeler: Auxin herbicides, Allium test, Comet assay, Chromosome aberrations, Mitotic index, DNA damage
- Uşak Üniversitesi Adresli: Evet
Özet
Clopyralid is one of the synthetic pyridine-carboxylate auxin herbicides and used to control perennial and annual broadleaf weeds in wheat, sugar beets, canola, etc. In this study, dose-dependent cytotoxicity and genotoxicity of clopyralid at different concentrations (25, 50, and 100 μg/mL) have been evaluated on the Allium cepa roots. The evaluation has been performed at macroscopic (root growth) and microscopic levels [mitotic index (MI), chromosome aberrations (CAs) in ana-telophase cells, and DNA damage] using root growth inhibition, Allium ana-telophase, and comet tests. The percentage of root growth inhibition and concentration of reducing root growth by 50% (EC50) of clopyralid were determined compared with the negative control by using various concentrations of clopyralid (6.25–1000 μg/L). The 96 h EC50 of clopyralid was recorded as 50 μg/L. The gradual decrease in root growth and the MI reveals the cytotoxic effects of clopyralid. All the tested concentrations of clopyralid induced total CAs (polyploidy, stickiness, anaphase bridges, chromosome laggards, and disturbed ana-telophase) and DNA damage dose and time dependently. These results confirm the cytotoxic and genotoxic effects of clopyralid on non-target organism.