Research Information System
Mediterranean Agricultural Sciences, vol.32, no.2, pp.193-199, 2019 (Peer-Reviewed Journal)
2-Chloropyridine (2-CPY) is an important precursor of cosmetics, pesticides and otherpharmaceutical products and it is also defined as trace chemical in industrial wastewater as theproducts of the metabolites of agricultural chemical products and river pollutants. In thisstudy, the effects of 2-CPY on mitotic index (MI), mitotic phase frequencies, chromosomeaberrations (CAs) and DNA damage in Allium cepa root cells were investigated with Alliumana-telophase and comet assay. Concentrations of 0.5xEC50 (25 ppm), EC50 (50 ppm) and2xEC50 (100 ppm) of 2-CPY, Methyl methanesulfonate (MMS-10 ppm, positive control) anddistilled water (negative control) were applied to A. cepa roots for 24, 48, 72 and 96 h. 2-CPYshowed a cytotoxic effect by reducing root growth and MI, but also showed genotoxic effectby increasing CAs (disturbed ana-telophase, chromosome laggards, stickiness, bridges andpolyploidy) and DNA damage at substantial levels. The amount of 2-CPY was shown to beincreased statistically in both duration and dose by liquid chromatography-tandem massspectrometry (LC-MS/MS). 2-CPY should be used carefully and investigated its cytogenotoxic effects with other toxicology test systems.