Antioxidant and Anti-Inflammatory Effects of Nicotinamide Adenine Dinucleotide (NAD+) Against Acute Hepatorenal Oxidative Injury in An Experimental Sepsis Model Deneysel Sepsis Modelinde Nikotinamid Adenin Dinükleotidin (NAD+ ) Akut Hepatorenal Oksidatif Hasara Karşı Antioksidan ve Anti-inflamatuar Etkileri

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Doganay S., Budak O., Sahin A., BAHTİYAR N.

Kafkas Universitesi Veteriner Fakultesi Dergisi, cilt.28, sa.1, ss.121-130, 2022 (SCI-Expanded, Scopus, TRDizin) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 28 Sayı: 1
  • Basım Tarihi: 2022
  • Doi Numarası: 10.9775/kvfd.2021.26609
  • Dergi Adı: Kafkas Universitesi Veteriner Fakultesi Dergisi
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, CAB Abstracts, EMBASE, Veterinary Science Database, Directory of Open Access Journals, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.121-130
  • Anahtar Kelimeler: Anti-inflammation, Hepatorenal injury, Nicotinamide, Oxidative stress, Sepsi
  • Açık Arşiv Koleksiyonu: AVESİS Açık Erişim Koleksiyonu
  • Uşak Üniversitesi Adresli: Evet

Özet

The aim of this study is to determine the antioxidant and anti-inflammatory effects of Nicotinamide Adenine Dinucleotide (NAD+) in preventing multi-organ damage caused by sepsis. Twenty-eight male Wistar-albino rats were randomly divided into four groups. The study groups comprised Sham group, sepsis group (CLP), sepsis + 100 mg/kg NAD+ (CLP+N100) and sepsis + 300 mg/kg NAD+ group (CLP+N300). Sepsis was induced by the cecum ligation perforation (CLP) method. NAD+ was administered intraperitoneally for five days before cecum perforation and 6 h after operation. Serum, liver and kidney tissues were taken from the rats 24 h after the operation. MDA, GSH, CAT, TNF-α, IL-6, IL-1β, and caspase-3 parameters were measured in tissue samples with biochemical and immunohistochemical methods. In the histopathological and immunohistochemical examination, increases in TNF-α, IL-6, IL-1β, and caspase-3 expressions were observed in the liver and kidney tissues of the CLP group and severe damage was seen in tissue morphology (P<0.001). Hepatorenal injury was significantly decreased in the treatment groups. Sepsis increased MDA levels in all tissues, but significantly decreased GSH and CAT activities. While NAD+ administration significantly increased GSH and CAT activity n the liver and kidney tissues, it caused a significant decrease in MDA levels. This study shows that nicotinamide may be a potent therapeutic agent for the treatment of sepsis.