Essential oil of ocimum gratissimum as a natural fungicide against pathogenic fungi of fruit crops and molecular docking studies
Brazilian Journal of Microbiology, cilt.57, sa.1, 2026 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 57 Sayı: 1
- Basım Tarihi: 2026
- Doi Numarası: 10.1007/s42770-026-01964-2
- Dergi Adı: Brazilian Journal of Microbiology
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, MEDLINE, Latin America & Iberia Database (ProQuest), Natural Science Collection (ProQuest), Biological Science Database (ProQuest), Health Research Premium Collection (ProQuest)
- Anahtar Kelimeler: Ocimum gratissimum, essential oil, fungal pathogens, molecular docking, biological activity
- Uşak Üniversitesi Adresli: Evet
Özet
Fungal diseases in fruit crops compromise productivity even when synthetic fungicides are applied. However, growing health and environmental concerns have made such products less attractive to consumers. In this context, the present study aimed to extract and characterise the essential oil (EO) from leaves and flowers of African basil (Ocimum gratissimum L.) by hydrodistillation and to evaluate its bioactivity against the fungi Botrytis cinerea, Fusarium guttiforme and Colletotrichum musae. Average EO yields were 3.90% w/w (leaves) and 2.60% w/w (flowers). Chromatographic analysis identified four constituents, mainly mono- and sesquiterpenes, with eugenol as the major compound (> 80%). Both the EO and pure eugenol displayed fungicidal activity against F. guttiforme and C. musae at 0.50 and 0.80 µL mL⁻¹ (EO) and at 0.50 and 1.20 µL mL⁻¹ (eugenol), respectively. The EO and eugenol were fungistatic against B. cinerea in vitro. The EO also inhibited more than 95% of C. musae conidial germination from 0.20 µL mL⁻¹. In vivo assays, the EO reduced anthracnose severity in bananas. Molecular docking studies revealed that germacrene D and (E)-caryophyllene exhibited the strongest binding affinities to the fungal CYP51 enzyme, with binding energies of − 7.6 and − 7.3 kcal/mol, respectively, indicating their potential as effective antifungal agents. These findings indicate that African basil EO is a promising biofungicide and could be strategically integrated into sustainable crop protection programmes.