Akademik Veri Yönetim Sistemi
10th Ecology And Evolutionary Biology Symposium, Çanakkale, Türkiye, 23 - 25 Temmuz 2024, ss.51
Weevils
(Coleoptera: Curculionoidea) constitute one of the most diverse groups of
insects, arranged in seven families (Anthribidae, Attelabidae, Belidae,
Brentidae, Caridae, Curculionidae and Nemonychidae) including approximately 62.000
described species. Modern phylogenetic classifications of weevils mostly agree
on family level, however; there is still some debate on relations of these
seven families. COI sequences i.e. DNA barcodes are widely used in species
identification as well as constructing hypothesis of evolutionary phylogenetic
relationships. This study aims to suggest and argue evolutionary phylogenetic
relationships between seven families of Curculionoidae by using COI sequences
available in NCBI database. We randomly
chose COI sequences which equally representing weevil family members and
constructed two datasets each including 32 COI sequences (five from each family,
two from Caridae which has only a few COI sequences in NCBI). We used two
Chrysomeloidae members as outgroup. We used MUSCLE algoritm to align sequences
and produced phylogenetic trees using Neighbor-Joining and Maximum Likelihood methods
in MEGA-X program. In all four phylogenetic trees produced, all families except
Caridae constituted monophyletic clades, however relationships of families
differed for two datasets and for the methods used. Basal and derived positions
of families within Curculionoidea were not identical in the four evolutionary
scenarios that resulted from the datasets we used. We argued the internal
relationships among families and compared our results with current phylogenies
in literature.