Relationships between glucose-6-phosphate dehydrogenase, glutathione peroxidase, reduced nicotinamide adenine dinucleotide phosphate, total protein, malondialdehyde, total glutathione and vitamin C parameters in goat milkcells

Creative Commons License

Kazak F., Karafakioğlu Y., Başpinar N., Coşkun P.

Journal of the Hellenic Veterinary Medical Society, cilt.73, ss.3699-3706, 2022 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 73
  • Basım Tarihi: 2022
  • Doi Numarası: 10.12681/jhvms.25512
  • Dergi Adı: Journal of the Hellenic Veterinary Medical Society
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, CAB Abstracts, EMBASE, Veterinary Science Database
  • Sayfa Sayıları: ss.3699-3706
  • Anahtar Kelimeler: G6PD, Goat milk cell, GPx, NADPH, tGSH
  • Uşak Üniversitesi Adresli: Evet

Özet

In this study, to reveal the antioxidant potential of goat milk cells, the activities of glucose-6-phosphate dehydrogenase (G6PD), glutathione peroxidase (GPx) and the levels of reduced nicotinamide adenine dinucleotide phosphate (NADPH), total glutathione (tGSH), malondialdehyde (MDA), vitamin C (Vit C) and total protein (TP) in goat milk cells were determined and correlations between these parameters were evaluated. Milk samples were collected from 19 clinically healthy goats from a private goat farm. Briefly, milk cells were collected from milk by centrifugation and than they were sonicated. Supernatant G6PD, GPx activities and NADPH, tGSH, MDA, Vit C and total protein levels were determined by spectrophotometric methods. As regards correlations: milk cell MDA levels were positively correlated with milk cell tGSH (r=0, 725, p<0, 01), milk cell Vit C (r=0.622, P<0.01) and milk cell NADPH (r=0.763, P<0.01) levels. There was a positive correlation between milk cell GPx activity and milk cell NADPH levels (r=0.659, P<0.01). Milk cell tGSH levels were positively correlated with milk cell Vit C (r=0.615, P<0.05) and milk cell NADPH (r=0.846, P<0.01) levels. Milk cell NADPH levels was positively correlated with milk cell Vit C levels (r=0.791, P<0.01). As a conclusion, the antioxidant potential of goat milk cells were evaluated and discusses.