The Synergistic Effect of Theranekron and Cisplatin on the Neuroblastoma (SH-SY5Y) Cell Line

TAŞPINAR N.

Turkish Neurosurgery, cilt.33, sa.6, ss.1113-1119, 2023 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 33 Sayı: 6
  • Basım Tarihi: 2023
  • Doi Numarası: 10.5137/1019-5149.jtn.43431-23.4
  • Dergi Adı: Turkish Neurosurgery
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, MEDLINE
  • Sayfa Sayıları: ss.1113-1119
  • Anahtar Kelimeler: Cisplatin, SH-SY5Y, Synergism, Theranekron
  • Uşak Üniversitesi Adresli: Evet

Özet

AIM: To assess the combined antitumor potential of theranekron (Trn) and cisplatin in neuroblastoma (NB), a pediatric tumor characterized by high prevalence and mortality rates. The investigation involved examining the interaction between theranekron (10-100 μM) and cisplatin (40 μM) within this cell line. MATERIAL and METHODS: The cytotoxicity of Trn was evaluated using MTT assays, cell volume analyses, apoptosis assessments, and measurements of mitochondrial membrane potential. These evaluations were conducted over a 24-hour incubation period. RESULTS: The coadministration of 100 μM Trn with cisplatin exhibited a cytotoxic effect of approximately 60%. Furthermore, it led to a reduction of up to 38% in cell volume. Notably, SH-SY5Y cells demonstrated an early apoptosis rate of 34.4%, accompanied by an eightfold decrease in mitochondrial membrane potential compared to the control group. CONCLUSION: Trn demonstrated synergism and significantly enhanced the efficacy of cisplatin (p<0.001). Nevertheless, further in-depth investigations are necessary to elucidate the intricacies of these synergistic effects.