Akademik Veri Yönetim Sistemi
Anais da Academia Brasileira de Ciencias, cilt.97, sa.4, 2025 (SCI-Expanded, Scopus)
Mentha pulegium L., a medicinal plant, has been known for producing bioactive essential oils (EOs) that exhibit antibacterial, antifebrile and digestive properties. Thus, this study aimed at determining, for the first time, activities of essential oil from Mentha pulegium (EO-MP) against Xylella fastidiosa - which causes Citrus Variegated Chlorosis (CVC) - and Xanthomonas citri - which causes citrus canker. Antibacterial activities of EO-MP were evaluated and the minimum inhibitory concentration (MIC) values were determined by the broth microdilution method in 96-well microplates. Cytotoxic activity of EO-MP was evaluated at concentrations ranging from 31.25 to 1000 µg/mL in tumor and non-tumor human cell lines. EO-MP was active against X. fastidiosa (MIC = 20 µg/mL) and X. citri (MIC = 50 µg/mL). EO-MP showed no cytotoxic activity to human fibroblast cell line (GM07492A) with IC50 equivalent to 1024.87 µg/mL and was potentially toxic against cell lines HeLa, MCF-7, and U-251MG (with all IC50 = 100 µg/mL). GC-MS analyses revealed that pulegone (51.3%), 1,8-cineole (16.5%), menthone (11.2%), and menthol (10.0%) were its major constituents in EO-MP. In silico studies highlighted that 1,8-cineole was more active against X. fastidiosa and pulegone against X. citri. In sum, further in vivo investigations and the ones conducted in a greenhouse are needed.