Synthesis and cytotoxic studies of novel azo-Schiff base derivatives against Hela and MCF7 cell lines

Guldu O. K., Yüksekdanacı S.

Journal of Chemical Sciences, vol.137, no.2, 2025 (SCI-Expanded) identifier

  • Publication Type: Article / Article
  • Volume: 137 Issue: 2
  • Publication Date: 2025
  • Doi Number: 10.1007/s12039-025-02364-z
  • Journal Name: Journal of Chemical Sciences
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, CAB Abstracts, Chemical Abstracts Core, Compendex
  • Keywords: apoptosis, cell migration assay, cell proliferation, Schiff bases
  • Uşak University Affiliated: Yes

Abstract

A new azo-Schiff base derivative compounds have been synthesized and characterized by FTIR, 1HNMR, 13CNMR and elemental analysis techniques. The azo-Schiff base compounds were evaluated for their cytotoxicity against human epithelial breast adenocarcinoma (MCF7) and human epithelial cervix adenocarcinoma (HeLa) cell lines using the MTT assay. Compound 4a exhibited potent in vitro antiproliferative activity with IC50 120.0 and 140.8 μM against HeLa and MCF7 cell lines, respectively. As a result of the cell migration study, compound 4a achieved 38%, recovery in HeLa cells at 48 hours and 59% in MCF7 cells at 48 hours. Compound 4b achieved 12% recovery in HeLa cells at 48 hours and 49% in MCF7 cells at 48 hours. Apoptosis analysis studies show that the synthesized compounds (4a-b) can induce apoptosis in the HeLa and MCF7 cells.