Genotoxicity of indium tin oxide by Allium and Comet tests

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Ciğerci İ. H., LİMAN R., Özgül E., Konuk M.

Cytotechnology, vol.67, no.1, pp.157-163, 2015 (SCI-Expanded, Scopus) identifier identifier

  • Publication Type: Article / Article
  • Volume: 67 Issue: 1
  • Publication Date: 2015
  • Doi Number: 10.1007/s10616-013-9673-0
  • Journal Name: Cytotechnology
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.157-163
  • Keywords: Allium test, Chromosome aberration, Comet assay, DNA damage
  • Open Archive Collection: AVESIS Open Access Collection
  • Uşak University Affiliated: Yes

Abstract

Genotoxic effects of indium tin oxide (ITO) were investigated on root cells of Allium cepa by employing both Allium and Comet assays. A. cepa roots were treated with the aqueous dispersions of ITO at 5 different concentrations (12.5, 25, 50, 75, and 100 ppm) for 4 h. Exposure of ITO significantly increased mitotic index, and total chromosomal aberrations by the Allium test. While chromosome laggards, stickiness, disturbed anaphase–telophase and anaphase bridges were observed in anaphase–telophase cells, c-metaphase and binuclear cells were observed in other cells. A significant increase in DNA damage was also observed at all concentrations of ITO by the Comet assay. These results indicate that ITO exhibits genotoxic activity in A. cepa root meristematic cells.