In vitro multiple shoot regeneration from Scilla siberica subsp. armena petiole Scilla siberica subsp. armena yaprak sapindan in vitro çoklu sürgün rejenerasyonu

Özdemir F. A., YILDIRIM M. U.

Yuzuncu Yil University Journal of Agricultural Sciences, vol.26, no.2, pp.215-220, 2016 (Scopus, TRDizin) identifier

  • Publication Type: Article / Article
  • Volume: 26 Issue: 2
  • Publication Date: 2016
  • Journal Name: Yuzuncu Yil University Journal of Agricultural Sciences
  • Journal Indexes: Scopus, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.215-220
  • Keywords: Explant, Geophyte, Propagation, Scilla siberica subsp. armena, Tissue culture
  • Uşak University Affiliated: Yes

Abstract

Scilla siberica subsp. armena is a perennial blue flowered bulbous plant. This study aimed to develop an efficient multiple shoot regeneration system for S. siberica subsp. armena using petiole on MS medium containing 0.25, 0.50, 1, 2 mg L-1 TDZ and 0.1, 0.2 mg L-1 NAA (8 combinations). Maximum mean shoot regeneration percentage was noted in MS medium containing 2 mg L-1 TDZ + 0.1 mg L-1 NAA, maximum mean number of shoot per explant was recorded in MS medium containing 2 mg L-1 TDZ + 0.2 mg L-1 NAA, maximum mean number of shoot length was recorded in MS medium supplemented with 0.25 mg L-1 TDZ + 0.2 mg L-1 NAA. Well developed micro propagated shoots were rooted on MS medium containing 1 mg L-1 IBA.