Studies on toxicity of noscapine loaded hydroxyapatite nanoparticles
Sigma Journal of Engineering and Natural Sciences, cilt.41, sa.4, ss.824-836, 2023 (ESCI, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 41 Sayı: 4
- Basım Tarihi: 2023
- Doi Numarası: 10.14744/sigma.2023.00090
- Dergi Adı: Sigma Journal of Engineering and Natural Sciences
- Derginin Tarandığı İndeksler: Emerging Sources Citation Index (ESCI), Scopus, Academic Search Premier, Directory of Open Access Journals
- Sayfa Sayıları: ss.824-836
- Uşak Üniversitesi Adresli: Evet
Özet
In this study, we loaded noscapine (NOS) into the hydroxyapatite nanoparticles (HAPs) and determine the release of NOS in acidic pH to extend its circulation time in the bloodstream. For the aim, NOS loaded HAP nanoparticles were obtained to characterize typical functional groups and to examine the size, the morphology of these nanoparticles, Fourier Transform Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM), and Energy Dispersive X-ray (EDX), Transmission Electron Microscopy (TEM) analysis were employed. Intercalary prepared HAPs are characterized by X-ray diffraction. The amount of NOS trapped in the HAPs was determined by High-performance liquid chromatography (HPLC). Comet Assay also evaluated the DNA damage of NOS-loaded hydroxyapatite nanoparticles (HAPs). The significance levels in different treatment groups were analyzed using the Duncan multiple range tests in SPSS 23.0 version for Windows software. P<0.05 was set as statistical significance. In the characterization studies, it was observed that NOS release increased linearly in acidity over time. The toxicities of the HAP and NOS-loaded HAP were also evaluated. NOS-loaded HAP was found to be reducing the harmful effects. Therefore, HAP seems to be an interesting alternative for further studies on noscapine transport and dose-dependent toxicity reduction.