Spectrophotometric determination of various polyanions with polymeric film optodes using microtiter plate reader


Dürüst N., Meyerhoff M. E., Ünal N., Naç S.

Analytica Chimica Acta, cilt.699, sa.1, ss.107-112, 2011 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 699 Sayı: 1
  • Basım Tarihi: 2011
  • Doi Numarası: 10.1016/j.aca.2011.05.007
  • Dergi Adı: Analytica Chimica Acta
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.107-112
  • Anahtar Kelimeler: Chromoionophore, Microtiter plate-format optode, Polyanion, Polymeric film sensor, Thickening agent
  • Uşak Üniversitesi Adresli: Hayır

Özet

Polycation-sensitive membrane optodes based on the chromoionophore 2′,7′-dichlorofluorescein octadecylester (DCFOE) have previously been developed and used for determination of heparin via a titrimetric method. In this study, it is shown that some other important polyanions such as PPS (pentosan polysulfate), DNA, xanthan, Na-alginate, and carrageenan (food additive) can also be readily determined by using DCFOE-based microtiter plate-format optodes (MPOs) and polycationic titrants that bind these polyanionic species. The optical sensors are prepared with poly(vinyl chloride) (PVC), polyurethane (PU), bis(2-ethylhexyl)sebacate (DOS), and 2′,7′-dichlorofluorescein octadecylester (DCFOE) and exhibit reproducible and sensitive absorbance changes in response to the varying polycationic titrant concentrations. Three different polycations; protamine, poly-l-lysine and poly-l-arginine, are employed as titrants. The method has a detection limit of 1μgmL-1, and a dynamic range of 1-40μgmL-1. After the quantitative determinations are successfully demonstrated in buffered solutions, similar titrations are also performed in real samples. The method is validated by recovery studies in these samples. The average polyanion recoveries were quantitative [99.7(±1.3) % for pastry cream with vanillin (protamine titrant); 100.4 (±3.3) % for pastry gel with strawberry(PLA titrant), and 102.9(±2.0) % for pastry gel with strawberry (PLL titrant)]. © 2011 Elsevier B.V.