Genetic variation in EMS-treated potato calli assessed by ISSR

ALTINDAL N., KARADOĞAN T., Altındal D.

Plant Cell, Tissue and Organ Culture, vol.163, no.2, 2025 (SCI-Expanded, Scopus) identifier

  • Publication Type: Article / Article
  • Volume: 163 Issue: 2
  • Publication Date: 2025
  • Doi Number: 10.1007/s11240-025-03265-2
  • Journal Name: Plant Cell, Tissue and Organ Culture
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, Compendex, Environment Index
  • Keywords: DNA markers, Mutation breeding, Polymorphism, Solanaceae, Somaclonal variation, Tissue culture
  • Uşak University Affiliated: Yes

Abstract

This study evaluated the genetic effects of EMS (ethyl methanesulfonate) treatments (0.1%–0.6% for 60–180 min) on plants regenerated from callus tissues of Solanum tuberosum L. cv. Agria. Callus formation occurred only in the Agria cultivar; no callus was observed in Marfona and Lady Olimpia. ISSR (Inter Simple Sequence Repeat) markers were used to assess genetic variation in EMS-treated regenerants. ISSR-PCR analysis revealed that lower EMS concentrations (0.1%–0.2%) with longer durations (180 min) preserved genetic similarity, while higher concentrations (0.6%) and shorter durations (60 min) increased genetic distance and polymorphism. PCA and UPGMA analyses supported a strong correlation between EMS dose/duration and genetic variability. These findings demonstrate the mutagenic efficiency of EMS in callus-based regeneration and highlight the high resolution of ISSR markers for detecting induced variation. The results contribute to mutation-based breeding strategies aiming to enhance genetic variation in potato.