Phylogenetic Relationships of The Trifolium L. Species Based on cpDNA Sequences


Yılmaz A., Yeltekin Y.

Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, vol.26, no.2, pp.299-306, 2023 (ESCI) identifier

Abstract

DNA barcoding is an important molecular approach in the determination of species diversity, evaluation of phylogenetic relationships and identification of taxonomically problematic species because of insufficient diagnostic characters. It has great importance to determine the barcoding regions that will give the best result in the evaluation of phylogenetic relationships in species like Trifolium that spread over wide geographical regions in the world and show high variation accordingly. For this aim, matK (maturase K) and rbcL (ribulose bisphosphate carboxylase gene) regions belonging to cpDNA(chloroplast DNA) was used to determine the barcoding ability and evaluate the taxonomy of the genus Trifolium. 63 taxa from matK region and 47 taxa from rbcL region were determined and used in this study. It was observed that transitional substitutions for matK and rbcL regions are higher than transversional substitutions with the rate of 51.52 % and 70.69 %, respectively. It can be stated that both of barcoding regions are valuable to reveal the phylogenetic relationships, in addition to their grouping ability the species as taxonomically. However, especially the using of matK sequence informations that have high variable sites (158) and grouping ability clearly for all taxa is strongly recommended.