The Evaluation of psbA-trnH IGS Sequences in The Genus Potentilla L. as Barcoding Region


YILMAZ A.

KSU TARIM VE DOGA DERGISI-KSU JOURNAL OF AGRICULTURE AND NATURE, vol.27, no.4, pp.773-781, 2024 (ESCI) identifier

Abstract

With more than 400 species, the genus Potentilla has a wide distribution causing morphological variations. Besides the wide distribution, many conditions such as hybridization, introgression, autopolyploidy, and allopolyploidy are observed in the genus. These can lead to misidentifications and taxonomic problems. DNA barcoding studies are important for species identification and solving taxonomic problems. However, preferred DNA sequences may have different effects among plant groups. Therefore, which DNA regions should be preferred is important to obtain more comprehensive results. In this research, psbAtrnH IGS sequences belonging to the Potentilla taxa were examined based on their compatibility and analyzed variable sites, passim-info sites, and nucleotide frequencies (%) for the region relevant by using Molecular Evolutionary Genetics Analysis (MEGA 11). Finally, a Maximum Parsimony (MP) dendrogram was performed to evaluate the Potentilla taxa taxonomically and phylogenetically. As a result of this study, it can be stated that the taxa belonging to the genus Potentilla were well grouped in the dendrogram and the use of psbA-trnH IGS sequences is strongly recommended for further studies.